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pcmv3 survivin expression plasmid  (Sino Biological)


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    Sino Biological pcmv3 survivin expression plasmid
    Cell death induction by 1H13-survivin/BIRC5-derived peptides targeted to the cytoplasm, mitochondria, or nucleus (A) The sequences added to the 1H13-BIRC5 peptide to target it to the: (1) cytoplasm, (2) mitochondria, or (3) nucleus. The underlined sequence represents the 1H13 peptide; in red, amino acids indicate D-amino acid substitutions. (B) A549 cells were incubated for 90 min with 5 μM FITC-labeled, nucleus-targeted peptide IH13-Nuc, and were also IF-stained with anti--SMAC, anti-IP3R or anti-GM130 antibodies, and stained with DAPI to visualize the mitochondria, ER, Golgi, and nucleus, respectively. Confocal microscope images are shown, with white arrows indicating peptide co-localization with the mitochondria (SMAC). Orange and yellow arrows indicate peptide presence in the nucleus and cytosol, respectively. (C) A549 cells were incubated with the mitochondria- or nucleus-targeted 1H13-BIRC5-derived peptide for 24 h in serum-free medium, followed by a cell proliferation assay using the SRB method. (D and E) Apoptotic cell death as induced in A549 cells following incubation for 24 h with the nucleus-targeted peptide (2/3D-1H13-Nuc) in the presence or absence of the indicated concentrations of the peptides in serum-free medium and subjected to FITC–annexin V/PI staining, followed by a flow cytometry analysis. Representative histograms for control and selected peptide concentration (D) and analysis of early and late apoptotic stages are shown (E). (F and G) Cell death as induced by 2/3D-1H13-Nuc in different cell lines, A549, SH-SY5Y, U-87MG, PC-3, and HUV-EC-C (F) or Jurkat, K562, and KMH2-LC (G) were treated with the indicated concentrations of the peptide for 24 h, then subjected to cell death analysis using propidium iodide (PI) staining and flow cytometry. (H and I) A549 cells were seeded at a density of 2 × 10 5 cells per well in a 12-well plate. After 24 h, the cells were transfected with 2 μg of <t>a</t> <t>pCMV3-survivin</t> expression plasmid <t>(HG10356-UT,</t> Sino Biological, China) or with an empty pCMV3 plasmid (control) using JetPrime transfection reagent (Polyplus, France), following the manufacturer’s instructions. Twenty-four hours post-transfection, the cells were re-seeded at 1 × 10 5 cells per well in a 12-well plate. After another 24 h, the culture medium was replaced with serum-free medium, and the cells were treated with the indicated concentrations of the 2/3D-1H13-Nuc peptide. Survivin overexpression levels were assessed by immunoblotting (H), and cell death was analyzed by propidium iodide (PI) staining followed by FACS analysis (I). Results represent the means ± SEM ( n = 3).
    Pcmv3 Survivin Expression Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pcmv3 survivin expression plasmid - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Survivin/BIRC5-derived peptide disrupts survivin dimerization and cell division and induces multifaceted anti-cancer effects"

    Article Title: Survivin/BIRC5-derived peptide disrupts survivin dimerization and cell division and induces multifaceted anti-cancer effects

    Journal: Molecular Therapy Oncology

    doi: 10.1016/j.omton.2025.201123

    Cell death induction by 1H13-survivin/BIRC5-derived peptides targeted to the cytoplasm, mitochondria, or nucleus (A) The sequences added to the 1H13-BIRC5 peptide to target it to the: (1) cytoplasm, (2) mitochondria, or (3) nucleus. The underlined sequence represents the 1H13 peptide; in red, amino acids indicate D-amino acid substitutions. (B) A549 cells were incubated for 90 min with 5 μM FITC-labeled, nucleus-targeted peptide IH13-Nuc, and were also IF-stained with anti--SMAC, anti-IP3R or anti-GM130 antibodies, and stained with DAPI to visualize the mitochondria, ER, Golgi, and nucleus, respectively. Confocal microscope images are shown, with white arrows indicating peptide co-localization with the mitochondria (SMAC). Orange and yellow arrows indicate peptide presence in the nucleus and cytosol, respectively. (C) A549 cells were incubated with the mitochondria- or nucleus-targeted 1H13-BIRC5-derived peptide for 24 h in serum-free medium, followed by a cell proliferation assay using the SRB method. (D and E) Apoptotic cell death as induced in A549 cells following incubation for 24 h with the nucleus-targeted peptide (2/3D-1H13-Nuc) in the presence or absence of the indicated concentrations of the peptides in serum-free medium and subjected to FITC–annexin V/PI staining, followed by a flow cytometry analysis. Representative histograms for control and selected peptide concentration (D) and analysis of early and late apoptotic stages are shown (E). (F and G) Cell death as induced by 2/3D-1H13-Nuc in different cell lines, A549, SH-SY5Y, U-87MG, PC-3, and HUV-EC-C (F) or Jurkat, K562, and KMH2-LC (G) were treated with the indicated concentrations of the peptide for 24 h, then subjected to cell death analysis using propidium iodide (PI) staining and flow cytometry. (H and I) A549 cells were seeded at a density of 2 × 10 5 cells per well in a 12-well plate. After 24 h, the cells were transfected with 2 μg of a pCMV3-survivin expression plasmid (HG10356-UT, Sino Biological, China) or with an empty pCMV3 plasmid (control) using JetPrime transfection reagent (Polyplus, France), following the manufacturer’s instructions. Twenty-four hours post-transfection, the cells were re-seeded at 1 × 10 5 cells per well in a 12-well plate. After another 24 h, the culture medium was replaced with serum-free medium, and the cells were treated with the indicated concentrations of the 2/3D-1H13-Nuc peptide. Survivin overexpression levels were assessed by immunoblotting (H), and cell death was analyzed by propidium iodide (PI) staining followed by FACS analysis (I). Results represent the means ± SEM ( n = 3).
    Figure Legend Snippet: Cell death induction by 1H13-survivin/BIRC5-derived peptides targeted to the cytoplasm, mitochondria, or nucleus (A) The sequences added to the 1H13-BIRC5 peptide to target it to the: (1) cytoplasm, (2) mitochondria, or (3) nucleus. The underlined sequence represents the 1H13 peptide; in red, amino acids indicate D-amino acid substitutions. (B) A549 cells were incubated for 90 min with 5 μM FITC-labeled, nucleus-targeted peptide IH13-Nuc, and were also IF-stained with anti--SMAC, anti-IP3R or anti-GM130 antibodies, and stained with DAPI to visualize the mitochondria, ER, Golgi, and nucleus, respectively. Confocal microscope images are shown, with white arrows indicating peptide co-localization with the mitochondria (SMAC). Orange and yellow arrows indicate peptide presence in the nucleus and cytosol, respectively. (C) A549 cells were incubated with the mitochondria- or nucleus-targeted 1H13-BIRC5-derived peptide for 24 h in serum-free medium, followed by a cell proliferation assay using the SRB method. (D and E) Apoptotic cell death as induced in A549 cells following incubation for 24 h with the nucleus-targeted peptide (2/3D-1H13-Nuc) in the presence or absence of the indicated concentrations of the peptides in serum-free medium and subjected to FITC–annexin V/PI staining, followed by a flow cytometry analysis. Representative histograms for control and selected peptide concentration (D) and analysis of early and late apoptotic stages are shown (E). (F and G) Cell death as induced by 2/3D-1H13-Nuc in different cell lines, A549, SH-SY5Y, U-87MG, PC-3, and HUV-EC-C (F) or Jurkat, K562, and KMH2-LC (G) were treated with the indicated concentrations of the peptide for 24 h, then subjected to cell death analysis using propidium iodide (PI) staining and flow cytometry. (H and I) A549 cells were seeded at a density of 2 × 10 5 cells per well in a 12-well plate. After 24 h, the cells were transfected with 2 μg of a pCMV3-survivin expression plasmid (HG10356-UT, Sino Biological, China) or with an empty pCMV3 plasmid (control) using JetPrime transfection reagent (Polyplus, France), following the manufacturer’s instructions. Twenty-four hours post-transfection, the cells were re-seeded at 1 × 10 5 cells per well in a 12-well plate. After another 24 h, the culture medium was replaced with serum-free medium, and the cells were treated with the indicated concentrations of the 2/3D-1H13-Nuc peptide. Survivin overexpression levels were assessed by immunoblotting (H), and cell death was analyzed by propidium iodide (PI) staining followed by FACS analysis (I). Results represent the means ± SEM ( n = 3).

    Techniques Used: Derivative Assay, Sequencing, Incubation, Labeling, Staining, Microscopy, Proliferation Assay, Flow Cytometry, Control, Concentration Assay, Transfection, Expressing, Plasmid Preparation, Over Expression, Western Blot



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    Cell death induction by 1H13-survivin/BIRC5-derived peptides targeted to the cytoplasm, mitochondria, or nucleus (A) The sequences added to the 1H13-BIRC5 peptide to target it to the: (1) cytoplasm, (2) mitochondria, or (3) nucleus. The underlined sequence represents the 1H13 peptide; in red, amino acids indicate D-amino acid substitutions. (B) A549 cells were incubated for 90 min with 5 μM FITC-labeled, nucleus-targeted peptide IH13-Nuc, and were also IF-stained with anti--SMAC, anti-IP3R or anti-GM130 antibodies, and stained with DAPI to visualize the mitochondria, ER, Golgi, and nucleus, respectively. Confocal microscope images are shown, with white arrows indicating peptide co-localization with the mitochondria (SMAC). Orange and yellow arrows indicate peptide presence in the nucleus and cytosol, respectively. (C) A549 cells were incubated with the mitochondria- or nucleus-targeted 1H13-BIRC5-derived peptide for 24 h in serum-free medium, followed by a cell proliferation assay using the SRB method. (D and E) Apoptotic cell death as induced in A549 cells following incubation for 24 h with the nucleus-targeted peptide (2/3D-1H13-Nuc) in the presence or absence of the indicated concentrations of the peptides in serum-free medium and subjected to FITC–annexin V/PI staining, followed by a flow cytometry analysis. Representative histograms for control and selected peptide concentration (D) and analysis of early and late apoptotic stages are shown (E). (F and G) Cell death as induced by 2/3D-1H13-Nuc in different cell lines, A549, SH-SY5Y, U-87MG, PC-3, and HUV-EC-C (F) or Jurkat, K562, and KMH2-LC (G) were treated with the indicated concentrations of the peptide for 24 h, then subjected to cell death analysis using propidium iodide (PI) staining and flow cytometry. (H and I) A549 cells were seeded at a density of 2 × 10 5 cells per well in a 12-well plate. After 24 h, the cells were transfected with 2 μg of <t>a</t> <t>pCMV3-survivin</t> expression plasmid <t>(HG10356-UT,</t> Sino Biological, China) or with an empty pCMV3 plasmid (control) using JetPrime transfection reagent (Polyplus, France), following the manufacturer’s instructions. Twenty-four hours post-transfection, the cells were re-seeded at 1 × 10 5 cells per well in a 12-well plate. After another 24 h, the culture medium was replaced with serum-free medium, and the cells were treated with the indicated concentrations of the 2/3D-1H13-Nuc peptide. Survivin overexpression levels were assessed by immunoblotting (H), and cell death was analyzed by propidium iodide (PI) staining followed by FACS analysis (I). Results represent the means ± SEM ( n = 3).
    Expression Plasmids, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    expression plasmids - by Bioz Stars, 2026-03
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    fluc gene orf cdna  (Sino Biological)
    94
    Sino Biological fluc gene orf cdna
    (a) Schematic illustration of the design of LNP and PLNP. (b) Left: sizes of LNP and PLNP measured by dynamic light scattering (DLS). Right: TEM images of LNP and PLNP. Scale bars: 100 nm. (c) Polydispersity index (PDI) of LNP and PLNP. (d) mRNA encapsulation efficiencies of LNP and PLNP. (e) Spike mRNA translation in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP or PLNP for 48 hours. Spike expression were measured by flow cytometry. Dose: 1 µg mRNA/mL. n = 3. (f) <t>FLuc</t> mRNA translation in RAW 264.7 macrophages on day 1, 2, 3, and 5 after incubation with FLuc mRNA-loaded LNP or PLNP. Dose: 1 µg mRNA/mL. n = 3. The FLuc signals were normalized to the day-1 signal of LNP-treated group. (g) Left: Representative flow plots of CD11c + DCs expressing SIINFEKL/H-2K b after incubation with OVA mRNA-loaded LNP or PLNP for 48 hours. Right: The percentages of CD11c + SIINFEKL/H-2K b+ DCs in total DCs. Dose: 1 µg mRNA/mL. n = 3. (h) Intracellular cytokine levels in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Left: IFN-γ. Middle: TNF-α. Right: IL-2. Dose: 1 µg mRNA/mL. n = 3. (i) Cytokine secretion from human PBMCs after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Dose: 1 µg mRNA/mL. n = 3. (j) Schematic illustration of PLNP enhancing antigen presentation and proinflammatory cytokine generation in APCs. Data are presented as mean ± s.e.m. Statistical significance was determined using two-sided unpaired t-test (d, e, g), one-way ANOVA with Tukey post hoc test for multiple comparisons (h), or two-way ANOVA with Tukey post hoc test for multiple comparisons ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.
    Fluc Gene Orf Cdna, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    fluc gene orf cdna - by Bioz Stars, 2026-03
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    full length desmin protein  (Sino Biological)
    94
    Sino Biological full length desmin protein
    (a) Schematic illustration of the design of LNP and PLNP. (b) Left: sizes of LNP and PLNP measured by dynamic light scattering (DLS). Right: TEM images of LNP and PLNP. Scale bars: 100 nm. (c) Polydispersity index (PDI) of LNP and PLNP. (d) mRNA encapsulation efficiencies of LNP and PLNP. (e) Spike mRNA translation in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP or PLNP for 48 hours. Spike expression were measured by flow cytometry. Dose: 1 µg mRNA/mL. n = 3. (f) <t>FLuc</t> mRNA translation in RAW 264.7 macrophages on day 1, 2, 3, and 5 after incubation with FLuc mRNA-loaded LNP or PLNP. Dose: 1 µg mRNA/mL. n = 3. The FLuc signals were normalized to the day-1 signal of LNP-treated group. (g) Left: Representative flow plots of CD11c + DCs expressing SIINFEKL/H-2K b after incubation with OVA mRNA-loaded LNP or PLNP for 48 hours. Right: The percentages of CD11c + SIINFEKL/H-2K b+ DCs in total DCs. Dose: 1 µg mRNA/mL. n = 3. (h) Intracellular cytokine levels in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Left: IFN-γ. Middle: TNF-α. Right: IL-2. Dose: 1 µg mRNA/mL. n = 3. (i) Cytokine secretion from human PBMCs after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Dose: 1 µg mRNA/mL. n = 3. (j) Schematic illustration of PLNP enhancing antigen presentation and proinflammatory cytokine generation in APCs. Data are presented as mean ± s.e.m. Statistical significance was determined using two-sided unpaired t-test (d, e, g), one-way ANOVA with Tukey post hoc test for multiple comparisons (h), or two-way ANOVA with Tukey post hoc test for multiple comparisons ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.
    Full Length Desmin Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length desmin protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    full length desmin protein - by Bioz Stars, 2026-03
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    Image Search Results


    Cell death induction by 1H13-survivin/BIRC5-derived peptides targeted to the cytoplasm, mitochondria, or nucleus (A) The sequences added to the 1H13-BIRC5 peptide to target it to the: (1) cytoplasm, (2) mitochondria, or (3) nucleus. The underlined sequence represents the 1H13 peptide; in red, amino acids indicate D-amino acid substitutions. (B) A549 cells were incubated for 90 min with 5 μM FITC-labeled, nucleus-targeted peptide IH13-Nuc, and were also IF-stained with anti--SMAC, anti-IP3R or anti-GM130 antibodies, and stained with DAPI to visualize the mitochondria, ER, Golgi, and nucleus, respectively. Confocal microscope images are shown, with white arrows indicating peptide co-localization with the mitochondria (SMAC). Orange and yellow arrows indicate peptide presence in the nucleus and cytosol, respectively. (C) A549 cells were incubated with the mitochondria- or nucleus-targeted 1H13-BIRC5-derived peptide for 24 h in serum-free medium, followed by a cell proliferation assay using the SRB method. (D and E) Apoptotic cell death as induced in A549 cells following incubation for 24 h with the nucleus-targeted peptide (2/3D-1H13-Nuc) in the presence or absence of the indicated concentrations of the peptides in serum-free medium and subjected to FITC–annexin V/PI staining, followed by a flow cytometry analysis. Representative histograms for control and selected peptide concentration (D) and analysis of early and late apoptotic stages are shown (E). (F and G) Cell death as induced by 2/3D-1H13-Nuc in different cell lines, A549, SH-SY5Y, U-87MG, PC-3, and HUV-EC-C (F) or Jurkat, K562, and KMH2-LC (G) were treated with the indicated concentrations of the peptide for 24 h, then subjected to cell death analysis using propidium iodide (PI) staining and flow cytometry. (H and I) A549 cells were seeded at a density of 2 × 10 5 cells per well in a 12-well plate. After 24 h, the cells were transfected with 2 μg of a pCMV3-survivin expression plasmid (HG10356-UT, Sino Biological, China) or with an empty pCMV3 plasmid (control) using JetPrime transfection reagent (Polyplus, France), following the manufacturer’s instructions. Twenty-four hours post-transfection, the cells were re-seeded at 1 × 10 5 cells per well in a 12-well plate. After another 24 h, the culture medium was replaced with serum-free medium, and the cells were treated with the indicated concentrations of the 2/3D-1H13-Nuc peptide. Survivin overexpression levels were assessed by immunoblotting (H), and cell death was analyzed by propidium iodide (PI) staining followed by FACS analysis (I). Results represent the means ± SEM ( n = 3).

    Journal: Molecular Therapy Oncology

    Article Title: Survivin/BIRC5-derived peptide disrupts survivin dimerization and cell division and induces multifaceted anti-cancer effects

    doi: 10.1016/j.omton.2025.201123

    Figure Lengend Snippet: Cell death induction by 1H13-survivin/BIRC5-derived peptides targeted to the cytoplasm, mitochondria, or nucleus (A) The sequences added to the 1H13-BIRC5 peptide to target it to the: (1) cytoplasm, (2) mitochondria, or (3) nucleus. The underlined sequence represents the 1H13 peptide; in red, amino acids indicate D-amino acid substitutions. (B) A549 cells were incubated for 90 min with 5 μM FITC-labeled, nucleus-targeted peptide IH13-Nuc, and were also IF-stained with anti--SMAC, anti-IP3R or anti-GM130 antibodies, and stained with DAPI to visualize the mitochondria, ER, Golgi, and nucleus, respectively. Confocal microscope images are shown, with white arrows indicating peptide co-localization with the mitochondria (SMAC). Orange and yellow arrows indicate peptide presence in the nucleus and cytosol, respectively. (C) A549 cells were incubated with the mitochondria- or nucleus-targeted 1H13-BIRC5-derived peptide for 24 h in serum-free medium, followed by a cell proliferation assay using the SRB method. (D and E) Apoptotic cell death as induced in A549 cells following incubation for 24 h with the nucleus-targeted peptide (2/3D-1H13-Nuc) in the presence or absence of the indicated concentrations of the peptides in serum-free medium and subjected to FITC–annexin V/PI staining, followed by a flow cytometry analysis. Representative histograms for control and selected peptide concentration (D) and analysis of early and late apoptotic stages are shown (E). (F and G) Cell death as induced by 2/3D-1H13-Nuc in different cell lines, A549, SH-SY5Y, U-87MG, PC-3, and HUV-EC-C (F) or Jurkat, K562, and KMH2-LC (G) were treated with the indicated concentrations of the peptide for 24 h, then subjected to cell death analysis using propidium iodide (PI) staining and flow cytometry. (H and I) A549 cells were seeded at a density of 2 × 10 5 cells per well in a 12-well plate. After 24 h, the cells were transfected with 2 μg of a pCMV3-survivin expression plasmid (HG10356-UT, Sino Biological, China) or with an empty pCMV3 plasmid (control) using JetPrime transfection reagent (Polyplus, France), following the manufacturer’s instructions. Twenty-four hours post-transfection, the cells were re-seeded at 1 × 10 5 cells per well in a 12-well plate. After another 24 h, the culture medium was replaced with serum-free medium, and the cells were treated with the indicated concentrations of the 2/3D-1H13-Nuc peptide. Survivin overexpression levels were assessed by immunoblotting (H), and cell death was analyzed by propidium iodide (PI) staining followed by FACS analysis (I). Results represent the means ± SEM ( n = 3).

    Article Snippet: After 24 h, the cells were transfected with 2 μg of a pCMV3-survivin expression plasmid (HG10356-UT, Sino Biological, China) or with an empty pCMV3 plasmid (control) using JetPrime transfection reagent (Polyplus, France), following the manufacturer’s instructions.

    Techniques: Derivative Assay, Sequencing, Incubation, Labeling, Staining, Microscopy, Proliferation Assay, Flow Cytometry, Control, Concentration Assay, Transfection, Expressing, Plasmid Preparation, Over Expression, Western Blot

    (a) Schematic illustration of the design of LNP and PLNP. (b) Left: sizes of LNP and PLNP measured by dynamic light scattering (DLS). Right: TEM images of LNP and PLNP. Scale bars: 100 nm. (c) Polydispersity index (PDI) of LNP and PLNP. (d) mRNA encapsulation efficiencies of LNP and PLNP. (e) Spike mRNA translation in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP or PLNP for 48 hours. Spike expression were measured by flow cytometry. Dose: 1 µg mRNA/mL. n = 3. (f) FLuc mRNA translation in RAW 264.7 macrophages on day 1, 2, 3, and 5 after incubation with FLuc mRNA-loaded LNP or PLNP. Dose: 1 µg mRNA/mL. n = 3. The FLuc signals were normalized to the day-1 signal of LNP-treated group. (g) Left: Representative flow plots of CD11c + DCs expressing SIINFEKL/H-2K b after incubation with OVA mRNA-loaded LNP or PLNP for 48 hours. Right: The percentages of CD11c + SIINFEKL/H-2K b+ DCs in total DCs. Dose: 1 µg mRNA/mL. n = 3. (h) Intracellular cytokine levels in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Left: IFN-γ. Middle: TNF-α. Right: IL-2. Dose: 1 µg mRNA/mL. n = 3. (i) Cytokine secretion from human PBMCs after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Dose: 1 µg mRNA/mL. n = 3. (j) Schematic illustration of PLNP enhancing antigen presentation and proinflammatory cytokine generation in APCs. Data are presented as mean ± s.e.m. Statistical significance was determined using two-sided unpaired t-test (d, e, g), one-way ANOVA with Tukey post hoc test for multiple comparisons (h), or two-way ANOVA with Tukey post hoc test for multiple comparisons ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

    Journal: bioRxiv

    Article Title: Polymer-lipid hybrid nanoparticle enhances mRNA delivery and T cell-mediated immunity

    doi: 10.64898/2026.01.22.701138

    Figure Lengend Snippet: (a) Schematic illustration of the design of LNP and PLNP. (b) Left: sizes of LNP and PLNP measured by dynamic light scattering (DLS). Right: TEM images of LNP and PLNP. Scale bars: 100 nm. (c) Polydispersity index (PDI) of LNP and PLNP. (d) mRNA encapsulation efficiencies of LNP and PLNP. (e) Spike mRNA translation in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP or PLNP for 48 hours. Spike expression were measured by flow cytometry. Dose: 1 µg mRNA/mL. n = 3. (f) FLuc mRNA translation in RAW 264.7 macrophages on day 1, 2, 3, and 5 after incubation with FLuc mRNA-loaded LNP or PLNP. Dose: 1 µg mRNA/mL. n = 3. The FLuc signals were normalized to the day-1 signal of LNP-treated group. (g) Left: Representative flow plots of CD11c + DCs expressing SIINFEKL/H-2K b after incubation with OVA mRNA-loaded LNP or PLNP for 48 hours. Right: The percentages of CD11c + SIINFEKL/H-2K b+ DCs in total DCs. Dose: 1 µg mRNA/mL. n = 3. (h) Intracellular cytokine levels in RAW 264.7 macrophages after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Left: IFN-γ. Middle: TNF-α. Right: IL-2. Dose: 1 µg mRNA/mL. n = 3. (i) Cytokine secretion from human PBMCs after incubation with spike mRNA-loaded LNP, spike mRNA-loaded PLNP, empty LNP, or empty PLNP for 24 hours. Dose: 1 µg mRNA/mL. n = 3. (j) Schematic illustration of PLNP enhancing antigen presentation and proinflammatory cytokine generation in APCs. Data are presented as mean ± s.e.m. Statistical significance was determined using two-sided unpaired t-test (d, e, g), one-way ANOVA with Tukey post hoc test for multiple comparisons (h), or two-way ANOVA with Tukey post hoc test for multiple comparisons ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

    Article Snippet: Wild-type SARS-CoV-2 spike encoding plasmid was obtained from the MacMaster lab. Omicron spike (B.1.1.529), influenza A H1N1 (A/Puerto Rico/8/1934) hemagglutinin (HA), and FLuc sequences were obtained from commercially available omicron spike, HA, and FLuc Gene ORF cDNA clone expression plasmids (Sino Biological #VG40835-UT, Sino Biological #VG11684-UT, and Addgene #101156) and inserted into the same plasmid backbone using Gibson assembly.

    Techniques: Encapsulation, Incubation, Expressing, Flow Cytometry, Immunopeptidomics

    (a) Left: In vivo luminescence images of BALB/c mice treated with 1×PBS, FLuc mRNA-loaded LNP, or FLuc mRNA-loaded PLNP at 4, 24, 48, 72, and 96 hours post administration. Right: In vivo luminescence signals measured by integration of total flux for each mouse. Dose: 5 µg mRNA/mouse. N = 5. (b) Left: Fluorescence images of lymph nodes collected from C57BL/6 mice at 24 h post vaccination with DiD-labeled spike mRNA-loaded LNP or PLNP. Right: Quantification of LNP and PLNP accumulation in lymph nodes at 24 h post vaccination. Dose: 10 µg mRNA/mouse. n = 4. (c) Innate immune cell activation (CD86 + ) at 7 days post boost vaccination. C57BL/6 mice were vaccinated with spike mRNA-loaded LNP or PLNP on day 0 and boosted with same doses on day 21. Dose: 10 µg mRNA/mouse. n = 5. Left: DCs. Middle: Macrophages. Right: Monocytes. (d) Schematic illustration of PLNP enhancing lymph node targeting and innate immune cell activation. Data are presented as mean ± s.e.m. Statistical significance was determined using two-way ANOVA with Tukey post hoc test for multiple comparisons (a) or one-way ANOVA with Tukey post hoc test for multiple comparisons (b, c). ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

    Journal: bioRxiv

    Article Title: Polymer-lipid hybrid nanoparticle enhances mRNA delivery and T cell-mediated immunity

    doi: 10.64898/2026.01.22.701138

    Figure Lengend Snippet: (a) Left: In vivo luminescence images of BALB/c mice treated with 1×PBS, FLuc mRNA-loaded LNP, or FLuc mRNA-loaded PLNP at 4, 24, 48, 72, and 96 hours post administration. Right: In vivo luminescence signals measured by integration of total flux for each mouse. Dose: 5 µg mRNA/mouse. N = 5. (b) Left: Fluorescence images of lymph nodes collected from C57BL/6 mice at 24 h post vaccination with DiD-labeled spike mRNA-loaded LNP or PLNP. Right: Quantification of LNP and PLNP accumulation in lymph nodes at 24 h post vaccination. Dose: 10 µg mRNA/mouse. n = 4. (c) Innate immune cell activation (CD86 + ) at 7 days post boost vaccination. C57BL/6 mice were vaccinated with spike mRNA-loaded LNP or PLNP on day 0 and boosted with same doses on day 21. Dose: 10 µg mRNA/mouse. n = 5. Left: DCs. Middle: Macrophages. Right: Monocytes. (d) Schematic illustration of PLNP enhancing lymph node targeting and innate immune cell activation. Data are presented as mean ± s.e.m. Statistical significance was determined using two-way ANOVA with Tukey post hoc test for multiple comparisons (a) or one-way ANOVA with Tukey post hoc test for multiple comparisons (b, c). ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

    Article Snippet: Wild-type SARS-CoV-2 spike encoding plasmid was obtained from the MacMaster lab. Omicron spike (B.1.1.529), influenza A H1N1 (A/Puerto Rico/8/1934) hemagglutinin (HA), and FLuc sequences were obtained from commercially available omicron spike, HA, and FLuc Gene ORF cDNA clone expression plasmids (Sino Biological #VG40835-UT, Sino Biological #VG11684-UT, and Addgene #101156) and inserted into the same plasmid backbone using Gibson assembly.

    Techniques: In Vivo, Fluorescence, Labeling, Activation Assay